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Matric Chemistry: Biochemistry, questions with answers

Practise 16 real Federal Board questions on Biochemistry from 2023 to 2025. See the answer and explanation as soon as you answer.

16real questions
4multiple choice
4exam sessions

About Biochemistry in Matric Chemistry

Biochemistry is one of the 11 main chapters in our Federal Board Matric Chemistry question bank (10th class), and about 4% of its real questions come from it. Tayyari Karo has 16 real questions on this chapter from 2023 to 2025; 4 are multiple choice and marked instantly, and the rest are short and long questions with a model answer. By difficulty: 9 easy, 7 medium.

Work through the questions below, then open the explanation for each one. Redo the ones you missed a few days later.

Try 8 real questions

These are real past-paper questions. Think of your answer first, then open the explanation.

2025 1st Annual · 10th · Biochemistry

Proteins are essential for the formation of:

  1. Protoplasm
  2. Cholesterol
  3. Dextrin
  4. Cellulose
Show answer and explanation

Answer: A. Protoplasm

Proteins are a major structural and functional component of protoplasm, the living material of cells.

2025 2nd Annual · 10th · Biochemistry

Which bonds are responsible for holding amino acids together in proteins?

  1. Hydrogen bonds
  2. Ionic bonds
  3. Peptide bonds
  4. Glycosidic bonds
Show answer and explanation

Answer: C. Peptide bonds

Amino acids are linked together by peptide bonds (formed via condensation reactions between the −COOH of one amino acid and −NH₂ of the next).

2024 Annual · 10th · Biochemistry

Molecules of amino acids join to form a protein by eliminating ________ molecule.

  1. Carbon dioxide
  2. Ammonia
  3. Water
  4. Alkyl group
Show answer and explanation

Answer: C. Water

Amino acids join via peptide bonds through a condensation reaction, releasing a water molecule for each bond formed.

2023 Annual · 10th · Biochemistry

________ is a good source of Protein.

  1. Water
  2. Meat
  3. Sodium chloride
  4. Spinach
Show answer and explanation

Answer: B. Meat

Meat is a rich dietary source of protein.

2025 1st Annual · 10th · Biochemistry

Enlist any three uses of lipids.

Show model answer

1. Serve as a concentrated source and store of energy in the body.
2. Provide insulation against cold and cushion/protect internal organs.
3. Are essential components of cell membranes and help in absorption of fat-soluble vitamins (A, D, E, K).

2025 2nd Annual · 10th · Biochemistry

Draw the structure of a typical amino acid. Use that structure to develop a tripeptide molecule. Show the location of peptide bonds also.

Show model answer

General amino acid structure: A central (alpha) carbon bonded to an amino group (−NH₂), a carboxyl group (−COOH), a hydrogen atom, and a variable side chain (R): H₂N−CH(R)−COOH.
Forming a tripeptide: Three amino acids (e.g. with side chains R₁, R₂, R₃) join together through condensation reactions, each releasing one water molecule, forming two peptide bonds:
H₂N−CH(R₁)−CO−NH−CH(R₂)−CO−NH−CH(R₃)−COOH
The peptide bonds (shown in bold, −CO−NH−) are located between the carboxyl carbon of one amino acid and the amino nitrogen of the next, formed with the loss of a water molecule at each linkage (2 peptide bonds link 3 amino acids into a tripeptide).

2024 Annual · 10th · Biochemistry

Identify any two functional groups found in Amino acid. Draw structure of an amino acid.

Show model answer

Two functional groups in an amino acid: (1) Amino group (−NH₂) and (2) Carboxyl group (−COOH).
Structure of a general amino acid: A central (alpha) carbon atom bonded to an amino group (−NH₂), a carboxyl group (−COOH), a hydrogen atom (−H), and a variable side chain (−R group): H₂N−CH(R)−COOH.

2023 Annual · 10th · Biochemistry

Discuss any three uses of lipids.

Show model answer

1. Lipids serve as a concentrated store of energy in the body (providing more energy per gram than carbohydrates or proteins).
2. They provide thermal insulation under the skin, helping regulate body temperature.
3. They are essential structural components of cell membranes (phospholipids) and help in the absorption of fat-soluble vitamins (A, D, E, K).

Sessions with Biochemistry questions

SessionQuestions
2025 1st Annual4
2025 2nd Annual3
2024 Annual5
2023 Annual4

Common questions

What does the Biochemistry chapter cover on this page?

16 real Federal Board (FBISE) Chemistry questions on Biochemistry from 2023 to 2025, for 10th class. Each one shows the session it came from.

Are the answers official?

No. We could not find an official FBISE marking scheme for these papers, so the answers and explanations are written by Tayyari Karo for practice. Check anything important with your teacher or textbook.

Is it free?

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